Fig 2

Fig 2 MYCN overexpressing SAP cells display an ectopic NCC gene expression signature.

A) Violin plots depicting crestin (left) and sox10 (right) expression from single cell datasets of sox10 derived cells at 24, 48, and 69 hpf. B,C,F,G) WICHCR performed using HCR probes against crestin (B,C) or sox10 (F,G) and with antibody against EGFP on 3 dpf dbh:EGFP (B,F) and dbh:EGFP-MYCN (C,G) larvae. Representative images of the SCG region reveal expression of the markers and their colocalized channel (coloc). D,H) Percentage of EGFP⁺ or EGFP-MYCN⁺ cells co-expressing crestin (D) or sox10 (H) in 3 dpf larvae. For crestin EGFP n = 8, EGFP-MYCN n = 11; for sox10 EGFP n = 7, EGFP-MYCN n = 10. E,I) Mean crestin (E) or sox10 (I) fluorescence intensity quantified in the SCG and normalized to dbh:EGFP average intensity at 3 dpf. For crestin EGFP n = 11, EGFP-MYCN n = 8; for sox10 EGFP n = 7, EGFP-MYCN n = 10. Markers: EGFP (green), crestin (magenta), sox10 (orange) and coloc channel (yellow). A (anterior), P (posterior), D (dorsal), V (ventral) axes shown in upper left corner. Scale bars = 10 μm. For all graphs * P<0.05.