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Fig. 2 Expression of her3 in wild-type and mib1 ta52 b mutant zebrafish embryos. In situ hybridization demonstrates the expression of her3 in wild-type siblings (left panels) and mib1 ta52 b mutant embryos (right panels) at the bud stage (A) and 24 h post-fertilization (hpf) (B). Images depict dorsal views with anterior to the top. fmb, fore-midbrain boundary; hb, hindbrain; ipd, inter-proneuronal domain; mb, midbrain; mhb, mid-hindbrain boundary; scn, spinal cord neurons. The expression levels of her3 were validated using quantitative real-time polymerase chain reaction (qRT-PCR), displayed at the bottom panels. Quantitative data are presented as mean ± standard error of mean (SEM). Statistical analysis was performed by Student?s t-test. All reactions were performed in triplicate for each sample. **, p < 0.01.