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Fig. 3 DSS and Wgx-50 have no effect on the growth and development of zebrafish. (A) The number of spontaneous movements per minute in zebrafish larvae at 30 hpe (n = 20). There was no significant effect observed after drug treatment. (B) The number of heartbeats every 20 s in zebrafish larvae at 48 hpe (n = 20). There was no significant effect observed after drug treatment. (C) The body length of zebrafish larvae at 96 hpe (n = 10/group). There was no significant effect observed after drug treatment. (D) The morphology of zebrafish at 96 hpf was captured using a stereomicroscope. Scale bar: 100 μm. (E) Zebrafish larval PMR behavior monitored by Viewpoint system (n = 16). (F) The experimental workflow of PMR is shown in the diagram. (G,H) Data analysis of zebrafish larval PMR behavior monitored by Viewpoint system (n = 16). There was a significant decrease in activity after drug treatment. (ns, not significant; **** p < 0.0001, one-way ANOVA.)