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Fig. 1 DSS promotes the recruitment of neutrophils and the expression of pro-inflammatory cytokines. (A) Tg(lyz:EGFP) zebrafish larvae (3 dpf) were exposed to different concentrations of DSS for three days. (B) Images display the chemical formula of DSS. (C) Images demonstrate the recruitment of neutrophils in the zebrafish intestinal injury model under fluorescence microscopy. White rectangles represent counting areas. Scale: 100 μm. (D) Shows the statistical quantification of neutrophil recruitment in the zebrafish intestinal injury model under control group and different concentrations of DSS treatment. The recruitment of neutrophils in the zebrafish intestine increases to varying degrees with different concentrations of DSS treatment. (E–H) Display the gene expression levels of pro-inflammatory cytokines il-1, il-6, cxcl8a, and tnf-α in zebrafish under control group and different DSS concentrations. The levels of pro-inflammatory cytokines in zebrafish increase to varying degrees with different concentrations of DSS treatment. All statistical analyses are compared to the control group (Con group). (ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 one-way ANOVA.)