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Fig. 3 Loss of slc4a7 leads to retinal abnormalities in zebrafish larvae. (A) Immunostaining for RCVRN in slc4a7-deficient and control AB zebrafish strains at 5 dpf. (B) The fluorescence imaging depicts retinal pigment epithelium cells and amacrine cells in slc4a7-deficient and control Tg (gad1b:mCherry) strain at 5 dpf. (C) The fluorescence imaging focusing on blood vessel endothelial cells in slc4a7-deficient and control Tg(kdrl:mCherry) strains at 5 dpf. (D) The fluorescence imaging illustrates Müller cells in slc4a7-deficient and control Tg (gfap:egfp) strains at 5 dpf. (E) Statistical results for normalized fluorescence intensity of RCVRN. (F,G) Statistical results for normalized fluorescence intensity of mCherry signal in amacrine cells (ACs), (F), and the RPE layer (G). (H) Statistical results for vascular network thickness surrounding photoreceptors. (I) Statistical results for the normalized fluorescence intensity of the eGFP signal in Müller cell layer. Scale bar = 50 μm. Bar plots are shown as the mean ± s.e.m. The t-test was performed between the two groups. * p < 0.05, *** p < 0.001, **** p < 0.0001. GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer.