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Fig. 4 Impaired social preference behaviors in adult mutants. (A) Heat map visualization of zebrafish trajectories in the shoaling test of 4 mpf adults (12 groups, 5 zebrafish per group), based on 15 min. (B) Graph showing the average inter-fish distance. (C) Diagram showing the average distance moved by each group. (D) Heat map visualization of zebrafish trajectories in the three-tank test, in a total 10 min period (n = 26 per group). (E,F) Graph showing the time (second) spent in social and middle zones. (G) Heat map visualization of zebrafish trajectories in the mirror test (n = 25 per group). (H) Graph showing the time (second) spent in mirror contact zone. Data are presented as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns: no significance.