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Fig. 3 Abnormal locomotor and thigmotaxis activities of adult mutants. (A) Graph showing the average velocity of individual control, adnpa?/?; adnpb?/?, and adnp2a?/?; adnp2b+/? adults (n = 28 per group) at 4 mpf, in a total 30 min period. (B) Graph showed the average total distance travelled by control, adnpa?/?; adnpb?/?, and adnp2a?/?; adnp2b+/? adults. (C) The representative traces of movements of control, adnpa?/?; adnpb?/?, and adnp2a?/?; adnp2b+/? adults. (D) Graph showing the time (second) spent in center zone of the indicated groups. (E) Representative swimming trajectories showing the stereotypical and repetitive behaviors of adnpa?/?; adnpb?/? adults. (F) quantification of panel (E). (G) Heat map visualization of zebrafish trajectories in the light/dark box test, in a total of 10 min period (n = 26 per group). (H) Graph showing the frequency of entering into light zone. (I) Heat map visualization of zebrafish trajectories in the novel tank test, in 10 min period (n = 30 per group). (J) Graph showing time spent in top zone of the indicated groups. Data are presented as mean ± SEM; * p < 0.05, ** p < 0.01, **** p < 0.0001. ns: no significance.