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Fig. 2 Impaired locomotor activity of mutant larvae in the open field test. (A) Locomotor activity of indicated larvae at 7 dpf (n = 68 per group). The average distance moved within each 1 min bin under constant illumination condition was plotted. The Y axis shows the normalized distance (millimeters) travelled by larvae in each 1 min bin. (B) Quantification of panel A. (C) Representative swimming trajectories of individual larvae of the indicated groups in the thigmotaxis test. The left side is a diagram of the observation area, the inner concentric circles are the ?center zone?, and the outer concentric circles are the ?periphery zone?. (D) Graph showing the time (second) in center zone spent by the indicated larvae types. (E) Light/dark test of the indicated groups at 7 dpf (n = 48 per group). The activity was recorded for 20 min, after 40 min of acclimation. Shown were based on a 30 min light/dark test, with three-min light/dark cycles (D1/L1, D2/L2, and D3/L3). D1: dark interval 1; L1: light interval 1. The average distance moved within each 1 min bin under light or dark conditions was plotted. Grey color was used to highlight the dark intervals. (F) Quantification of panel E. The Y axis shows the normalized distance (millimeters) travelled by larvae in each 1 min bin. Data are presented as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ns: no significance.