|
Fig. 4 Indomethacin exposure induces the regulation of cardiac development-related genes in zebrafish embryos. (A) The representative images of several key cardiac development-related genes in cardiac regions of zebrafish embryos using WISH assay after 96 h indomethacin exposure. (B) The determination of mRNA levels of cardiac development-related genes in zebrafish embryos treated with different concentrations of indomethacin after 96 h of exposure using qPCR assay. Broken line indicates the relative expression levels of target gene in control groups. (C) Western blotting analysis of Myh6, Myh7, and Tpm1 expression in zebrafish embryos treated with different concentrations of indomethacin for 96 h (n = 100 for each group). All the experiments were performed independently with three biological replicates. All the values are presented as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.