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Fig. 3 Enhanced locomotion behavior during bPAC stimulation in cpe and tac1 knockouts (A) cpe homozygous knockouts and wild-type siblings (4 dpf) in the Tg[mnx1:Gal4]/Tg[UAS:bPAC-V2A-mCherry] double transgenic background were subjected to a swimming assay and swimming speed measured during blue light stimulation as indicated. Values are normalized to the first 2.5 s before illumination. Two-way ANOVA with Tukey multiple comparisons of means. (B) Swimming speed of tac1 homozygous knockouts and respective wild-type siblings (4 dpf) carrying the Tg[mnx1:Gal4]/Tg[UAS:bPAC-V2A-mCherry] transgenes before, during, and after bPAC activation. Values are normalized to the first 2.5 s before illumination. Two-way ANOVA with Tukey multiple comparisons of means. Data are displayed as means ± SEM. Statistical significance given as ???p < 0.001, ??p < 0.01, ?p < 0.05.