ZFIN Image: Bao et al., 2024, Fig. 1

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Figure Caption

Fig. 1 Nucleolar localization of human MPP10, UTP25/DEF, UTP13/TBL3, UTP12/WDR3 or EMG1 is UTP3/SAS10-dependent. (A) Nucleolar localization of human MPP10 is UTP3-dependent. HeLa cells were transfected with FLAG-tagged MPP10 plasmid alone or together with HA-tagged UTP3 plasmid for 48 h and were then subjected to immunofluorescence staining (IF) using the FLAG, MPP10 or HA antibodies as indicated. Scale bar, 5 μm. (B) Outline of the flowchart of the screening for SSU processome components (SSU_PCs) whose nucleolar localization is UTP3-dependent. (C–G) Showing the IF images of NOP56 representing the group I proteins (C), GFM1 representing the group II proteins (D), FAF1 representing the group III proteins (E), UTP25, UTP12 and EMG1 (group VI) (F), and UTP13 (group V) (G) expressed alone or together with UTP3 in HeLa cells 48 h post-transfection. IF was performed using FLAG, HA or UTP3 antibodies as indicated. DAPI (blue), staining the nuclei.

Acknowledgments

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