Fig. 5

Fig. 5 hdac6 can rescue the ciliopathy-like phenotype of ccdc141 crispants. A: Protein expression of acetylated-?-tubulin (Ace-?-Tub) and ?-tubulin (?-Tub) in zebrafishes of the control and ccdc141 crispants group was evaluated by Western blot. GAPDH served as a loading control. B: Quantification of acetylated-?-tubulin protein level when normalized against ?-tubulin. N = 3; ???, P < 0.001; Student's t-test. A.U, arbitrary units. C: The quantification of cardiac looping in zebrafish treated by DMSO (control) or different pharmaceuticals. Trichostatin A (TSA), a pan-HDAC inhibitor; Nicotinamide (NIC), an inhibitor of sirt1 and sirt2; Tubacin, a highly selective HDAC6 inhibitor. N = 3; n = 206/244/256/100/78/228. ????, P < 0.0001; ?, P < 0.05; ns, P > 0.05; one-way ANOVA test. D?F: The abnormal cardiac looping, pericardial edema, and curved tail in the ccdc141 crispants embryos under the inhibition of hdac6. N = 3; n = 206/244/266; ????, P < 0.0001; Fisher's exact test (D). N = 5/6; ????, P < 0.0001; ?, P < 0.05; ns, P > 0.05; one-way ANOVA test (E and F). G: Tubacin was able to partially rescue the spaw expression pattern in ccdc141 crispants. n = 67/39; ?, P < 0.05; two sided Fisher's exact test.