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Fig. 3 Neddylation enhances YAP-mediated signaling. A, 8xGTIIC-Luc reporter activity in Myc empty vector (200 ng) or Myc-YAP1 (200 ng) transfected HEK293T cells with or without HA-NEDD8 for 36 h. B, 8xGTIIC-Luc reporter activity in Myc empty vector (200 ng) or Myc-YAP1 (200 ng) transfected HEK293T cells with or without MLN4924 (1 ?M) treatment for 24 h. C, quantitative real-time PCR (qPCR) analysis of genes either positively or negatively regulated by YAP1 in rabbit GCs treated with or without MLN4924 (1 ?M) for 24 h. Four separate experiments were performed, with each measurement performed at least three times. Data are expressed as mean ± SD. (unpaired two-tailed Student's t test). ?p < 0.05, ??p < 0.01, and ???p < 0.001. GC, granulosa cell; ns, not significant; YAP1, yes-associated protein 1.