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Fig. 3 Hypoxia mimetics regulate the stability of an FLT4/ FLT4-TOF-PTV. RNA (A) or protein (B) was prepared from primary human ECs (HUVECs) expressing the FLT4 minigene assay construct (as outlined in Supplementary material online, Figure S4). EV, WT, or Q736* PTV FLT4 minigenes were untreated, or treated with the hypoxia mimetics, 0.2 mM cobalt chloride (CC) or 100 nM DMOG, for 3 h. Red dagger, full-length minigene protein, both V5 and FLAG positive; blue dagger, truncated minigene protein caused by the introduction of a nonsense codon and concomitant C-terminal cleavage, V5 positive only; n = 3; **P < 0.01; ****P < 0.0001.