|
Fig. 2 Cardiac progenitor specification is unaffected in gata6 mutants. (A,B) Immunostaining of 33 hpf nkx2.5:ZsYellow sibling (A) or gata6 mutant (B) embryos indicated normal formation of cardiac precursors (dorsal view). Scale bars: 50 µm. (C-E) The gata6 mutant hearts exhibit small but insignificant accumulation of Nkx2.5:ZsYellow progenitors within pharyngeal arch 2. Representative images of the 2nd pharyngeal arch (white arrows) in 30 hpf Tg(nkx2.5:ZsYellow) sibling (C) or gata6−/− (D) embryos. Scale bars: 20 μm. (E) Quantification of the area of ZsYellow expression within the 2nd arch indicates a slight increase in ZsYellow+ cells in gata6 mutants compared with siblings (ns, not significant by paired t-test, data are mean±s.e.m.). Area of signal was quantified by threshold using FIJI software.