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Fig. 4 Normal morphology of Meckel’s cartilage depends on IGF signaling. (A,B) Representative images of Alcian blue-stained wild-type zebrafish larvae treated with the IGF-IR inhibitor NVP-AEW541 (4 µM) or control solution (DMSO in E3) from 3 to 4 dpf. A magnification of one side of Meckel’s cartilage and a schematic outline of the chondrocytes in Meckel’s cartilage are also shown. (C) Length of Meckel’s cartilage after control (n = 8) and NVP-AEW541 (n = 8) treatment. Data are from two independent experiments. (D–F) A similar experiment using the structurally distinct IGF-IR inhibitor BMS-75807 (0.45 µM, n = 9; control group, n = 9). Data are from three independent experiments. Results are shown as mean ± SD, and are analyzed by Student’s t-test. ****p < 0.0001.