ZFIN Image: Marques et al., 2024, Figure 8

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Figure 8

Zebrafish were treated with 40 µM SFN or 1 µM tBHQ for 6 h during the pharyngula, hatching, and protruding-mouth stages and then collected for gene expression via quantitative real-time PCR. mRNA expression was measured for glutathione S-transferase Pi (gstp) along with the housekeeping gene β2-Microglobulin (b2m). The ΔΔCT method was used to calculate fold change. Values are mean fold change ± SEM. Statistical significance was assessed via two-way ANOVA followed by Fisher’s LSD post hoc test. n = 2–3 pools of 15–20 (hatching and protruding-mouth stages) or 20–40 (pharyngula) fish. Different letters indicate significant differences (p ≤ 0.05) among groups.

Acknowledgments

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