Figure 5

Figure 5

(A) Zebrafish were treated with 40 µM SFN or 1 µM tBHQ during the pharyngula, hatching, and protruding-mouth stages for 6 h and incubated with BioGee for 2 h prior to fixation (24 h post-exposure). BioGee protein conjugates were labeled in situ using immunohistochemistry. (B) Mean fluorescence intensity (Fl) of BioGee protein conjugates. Representative heatmaps were generated to visualize BioGee-protein conjugate fluorescence at the (C) pharyngula/hatching, (D) hatching/protruding-mouth, and (E) protruding-mouth/larval stages. Statistics were performed using a two-way ANOVA followed by Fisher’s LSD post hoc test. n = 8–10 fish. Different letters indicate significant differences (p ≤ 0.05) among groups. Scale bar = 100 μm.