Figure 1

Figure 1

(A) Zebrafish were treated with 40 µM SFN or 1 µM tBHQ during the pharyngula, hatching, and protruding-mouth stages for 6 h and then fixed, and Nrf2a protein was labeled using immunohistochemistry (IHC). The mean fluorescence intensity (FI) of Nrf2a protein in the (B) body tissue, (C) brain, (D) heart, (E) gut, and (F) pancreas was measured; liver data are presented at higher magnification in Figure 3 below. Representative heatmaps were generated to visualize Nrf2a protein fluorescence differences at the (G) pharyngula, (H) hatching, and (I) protruding-mouth stages. The gut and pancreas are not present (n.p.) at the pharyngula stage. Statistics were performed with a two-way ANOVA and Fisher’s LSD post hoc test. n = 7–12 fish. Letters indicate significant differences (p ≤ 0.05) among groups. * p ≤ 0.05, *** p ≤ 0.001, **** p ≤ 0.0001. Scale bar = 100 µm.