|
Fig. 3 Time course of the expression of MHB-related genes in embryos in which en-pou5f3 was induced during early somitogenesis. Embryos obtained by crosses of en-pou5f3+/− and wild-type fish were subjected to heat shock at 3-ss (11 hpf) and examined for expression of MHB-forming genes (A, pax2a; B, fgf8a; C, en2a) by WISH analysis from 1 to 10/14 hphs. The expression of egr2b in r3 and r5 of the hindbrain was also examined. Dorsal views are shown with the anterior to the top. Solid and open arrowheads mark normal and reduced expression at the MHB, respectively. For all marker genes and genotypes, at least 6 embryos were examined, all of which showed the indicated patterns. Additional data are shown in Fig. S2 . r3/r5, rhombomeres 3 and 5. os, optic stalk. Scale bars, 100 μm.