Figure 2

Figure 2 Chondrocyte cell death and proliferation in psmb1 mutants.

(A) Time-course study of apoptag TUNEL staining (green) and Sox9a staining (magenta) from 55–72 hpf. (B–E) Quantification of A demonstrates that cell death is not elevated in mutant chondrocytes until 72 hpf, at which point sox9a⁺TUNEL⁺ cells can be found (white arrowhead). 55 hpf: n = 10 (+/+), 16 (+/–), 6 (–/–). 60 hpf: n = 10 (+/+), 21 (+/–), 8 (–/–). 65 hpf: n = 6 (+/+), 6 (+/–), 6 (–/–). 72 hpf: n = 8 (+/+), 10 (+/–), 10 (–/–). (F) pH3 (magenta) antibody staining in sox10:kaede larvae (green) at 57 hpf, 65 hpf, and 72 hpf. (G–I) Quantification of C shows no proliferation of craniofacial chondrocytes across genotypes. 57 hpf: n = 6 (+/+), 10 (+/–), 6 (–/–). 65 hpf: n = 10 (+/+), 14 (+/–), 7 (–/–). 72 hpf: n = 7 (+/+), 11 (+/–), 9 (–/–). Scale bars: 50 μm. Data shown represent mean ± SD. Significance was calculated with 1-way ANOVA with Dunnett’s multiple comparisons test. *P < 0.05; ns, not significant.