Figure 6

Figure 6

Ca²⁺ dynamics in siBCL2L13 and siControl C2C12 myotubes

(A) 300 s confocal time-lapse recording of cytosolic Ca²⁺ levels.

(B) Quantification of normalized Fluo-4 fluorescence in response to 2.5 mM caffeine.

(C) 300 s confocal time-lapse recording of mitochondrial Ca²⁺ levels.

(D) Normalized Rhod-2 fluorescence in response to 1 μM thapsigargin. (A–D) n = 4 independent transfections per condition. (B and D) peak fluorescence was normalized to baseline. Error bars are mean ± SEM, ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗∗p < 0.0001 (unpaired t test).

See also Figure S5.