Figure 2

Figure 2

Skeletal muscle structure of WT and KO fish

(A) Representative immunohistochemistry of transverse muscle sections of WT and KO fish stained with phalloidin (red). Slow fibers are marked with an antibody against MYH7B (green, merge is yellow).

(B) Mean CSA of fast muscle fibers per fish.

(C) Mean number of fast fibers per ROI per fish.

(D) Mean CSA of slow muscle fibers per fish.

(E) Mean number of slow fibers per ROI per fish.

(F) Representative electron micrographs of fast muscle fibers of WT and KO fish.

(G) Contingency graph for healthy fibers vs. fibers with SR accumulation. For (C–E) n = 6 fish per group. Error bars are mean ± SEM, ^∗p < 0.05 (unpaired t test). For (G) n = 8 fish per group. p = 0.0007 (Fisher’s exact test). Squares (white in A, black in F) represent the regions that are zoomed out. WT: wild type and KO: Mutant bcl2l13. CSA is cross-sectional area; ROI is region of interest.