Fig. 3

Fig. 3 Ocular biometric parameters of control and 2-week dark-reared fish at 0, 1 and 2 weeks (w) post-recovery. (A) For rearing treatments, wild-type fish were reared under a constant dark environment for 2 weeks from 4 weeks post-fertilization (wpf), followed by recovery in normal light/dark rearing. (B–I) Parameters including (B) retinal radius to lens radius ratio, (C) equatorial diameter to lens radius ratio and (D) axial length to equatorial diameter ratio, as well as (E) axial length, (F) lens radius, (G) retinal radius, (H) equatorial diameter and (I) averaged corneal curvature, were compared between control and treated fish at 0 (baseline), 1 and 2 weeks of recovery. Violin plots show data for individual retinae with thick bars indicating medians, and thin lines indicating interquartile ranges. N = 40 control fish for each timepoint. N = 38, 40 and 37 dark-reared fish for 0, 1 and 2 w of recovery, respectively. Two-way ANOVA was performed. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Asterisks next to the vertical bars indicate the group effect, those above the horizontal bracket indicate Fisher's LSD post-hoc comparisons. Given that differences between groups for the tested parameters, if any, were in relatively small ranges, for better visualization, y-axes of figures may not start from 0.