Figure S9.

Figure S9. Hematoxylin and eosin photomicrographs of liver and pancreas, and double immunohistochemistry images of the pancreas of Mocs2^+/− mice.

(A, B, C, D, E, F) Images showing no abnormalities or differences in any examined tissue between Mocs2^+/+ and Mocs2^+/− male mice. (A, B, D, E) Representative H&E brightfield images from liver and pancreas from Mocs2^+/+ (A, B) and Mocs2^+/− (D, E) male mice. (C, F) Immunohistochemical double staining of the endocrine pancreas to assess islet morphology, showing insulin-producing (Ins, red) and glucagon-producing (Gcg, brown) cells in Mocs2^+/+ (C) and Mocs2^+/− (F) male mice. The morphology was similar to that of female mice. Scale bars are indicated in the figure. (G) Quantification of the INS-positive area as the percentage of the total pancreas area. Data are presented as mean values ± SEM. n = 4.