Figure S4.

Figure S4. Sodium molybdate induces the β-cell proliferation marker ins:venus-geminin but does not impact proliferation of other islet cell types.

(A, B, C) Single-plane confocal images of pancreatic islets in 6 dpf Tg(ins:flag-NTR); Tg(ins:venus-geminin) larvae after β-cell ablation and control (A) or sodium molybdate treatment (B) for 48 h. The nuclei are counterstained with TO-PRO-3. (C) Quantification of proliferating, ins:venus-geminin⁺, β-cells (C). Four independent experiments are shown with each independent experiment color-coded separately. Scale bar, 10 μm. n = 53–61. *P = 0.0231, non-parametric Mann–Whitney test. Data are presented as mean values ± SEM. (D, E, F) Single-plane confocal images of pancreatic islets in Tg(ins:H2BGFP) larvae after control (D) or sodium molybdate treatment (E) for 48 h, in the presence of EdU. (F) Quantification of total number and proliferating, ins:H2BGFP⁺EdU⁺, β-cells (F). Representative experiment from three biological replicates. Scale bar, 10 μm. n = 13–15. Data are presented as mean values ± SEM. (G, H, I, J) Single-plane confocal images of pancreatic islets in Tg(ins:flag-NTR) larvae after control (G) or sodium molybdate treatment (J) for 48 h, in the presence of EdU, after β-cell ablation, counterstained with Gcg and Sst. (I, J) Quantification of proliferating Gcg⁺EdU⁺ α-cells (I) and Sst⁺EdU⁺ δ-cells (J). Representative experiment of two biological replicates. Scale bar, 10 μm. n = 11–12. Data are presented as mean values ± SEM.