Fig. 3 Satellite glial cell ablation with central branch ablation improves central branch regrowth rate. (a) Diagram of dorsal root ganglion (DRG) central branch ablation (CBA) and satellite glia ablation (SGC-Abl) with CBA laser injury paradigms. (b) Percent of DRGs with successful and unsuccessful central branch regrowth into the dorsal spinal cord following CBA (n = 5/28 DRGs from 18 fish) with or without SGC-Abl (n = 9/17 DRGs from 14 fish; Pearson's ?² test, p = .033). (c) In vivo, time-lapse imaging of a Tg(ngn1:gfp;sox10:nls-eos) DRG at 3 dpf before (pre-injury) and after SGC-Abl CBA (?0 h postfertilization, hpf) with fluorescently labeled sensory neuron (asterisk; new dorsal neurite indicated by arrow) and SGC nuclei (white arrowheads). Spinal cord boundary indicated by dashed line. Single Z-plane inset indicated by square outline. Scale bars: 10 ?m. *p < .05.
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