ZFIN Image: Zhang et al., 2024, FIGURE 1

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Figure Caption

FIGURE 1

Hypoxic setup for larval zebrafish. (A) Pattern of hypoxic devices. 1 and 2, air diffuser; 3 and 4, oxygen electrode; 5–8, conduit connector. (B, C) The oxygen concentration inside the bottles were recalibrated to 20% air saturation (1.56 ppm, 1.6 mg/L) at each 12-h intervals (shown in B is not 12 h interval) from 1 to 5 dpf. (D) qRT-PCR analysis showed that the expression of hif1α was significantly increased after exposed to hypoxia. (E, F) Western blots showed that Hif1α protein expression was significantly increased under hypoxia. Hif1α, 110 kD; β-actin, 42 kD. *p < 0.05; **p < 0.01; ***p < 0.001.

Acknowledgments

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