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Fig. 2 OCLN-derived EL2 peptide inhibits monocyte transmigration across the BBB-like endothelium. (A) Schematic representation of OCLN (green) at the PM with OCLN-derived peptides highlighted in red (see sequence in Methods). (B) Permeability of hCMEC/D3 monolayer after overnight exposure of the monolayer to OCLN-derived peptides or their scramble controls. The data were obtained from n = 3 individual experiments. (C) Adhesion of primary monocytes on fibronectin after pre-incubation with OCLN-derived peptides or their scramble controls for 1 h measured with CellTiter Glo. Adhesion time is 30 min. The data were obtained from n = 4 donors in 2 individual experiments. Data were normalized to DMSO for each experiment. (D) Percentage of primary monocytes after 1 h exposure to OCLN-derived peptides or their scramble controls that transmigrated across the hCMEC/D3 monolayer overnight. The data were obtained from n = 8 individual experiments using 13 donors and normalized to the average DMSO for each experiment. Data information: In (B?D), data are presented as mean ± SEM. Two-tailed Student?s t-test p value < 0.05 (*), <0.01 (**), <0.001 (***) or non-significant (ns). Source data are available online for this figure.