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Fig. 6 Overexpression of lamp2a rescues the impaired definitive hematopoiesis in tmem106bb KO embryos. (A) Injection of lamp2a mRNA (mCherry RNA as control) rescued the reduced signal of cd41+ HSPCs in CHT of 3 dpf tmem106bb−/−/Tg(cd41:GFP) embryos (n = 29 embryos per group; scale bar: 200 μm). (B) WISH for rag1 showed that injection of lamp2a mRNA (mCherry RNA as control) rescued the decreased lymphoid differentiation in tmem106bb morphants (n = 19–20 embryos per group; scale bar: 200 μm). (C) WISH for mpx showed that injection of lamp2a mRNA (mCherry RNA as control) reversed the increased myeloid differentiation in tmem106bb morphants (n = 17–22 embryos per group; scale bar: 200 μm). (D) WISH for lplastin showed that injection of lamp2a mRNA (mCherry RNA as control) reversed the increased myeloid differentiation in tmem106bb morphants (n = 18–21 embryos per group; scale bar: 200 μm). (E) WISH for gata1 showed that injection of lamp2a mRNA (mCherry RNA as control) reversed the increased erythroid differentiation in tmem106bb morphants (n = 16-embryos per group; scale bar: 200 μm). (F) WISH for hbbe1 showed that injection of lamp2a mRNA (mCherry RNA as control) reversed the increased erythroid differentiation in tmem106bb morphants (n = 16-embryos per group; scale bar: 200 μm). *p < .05, **p < .01, ***p < .001, ns, no significance; Data were shown as mean ± SD; Statistical analysis was performed using a Shapiro–Wilk test for samples with normal distribution and two-way anova followed by a Sidak correction post hoc test.