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Fig. 1 Generation and characterization of tmem106bb KO zebrafish using CRISPR/Cas9 genome editing. (A) Strategy of CRISPR/Cas9 editing of tmem106bb with a site-specific targeting sgRNA for CRISPR/Cas9 cleavage in exon 3. Genomic DNA sequence analysis identified a mutant zebrafish line with a 7-bp deletion on exon 3, a frameshift mutation that results in a truncated Tmem106b protein with N-terminal 121 amino acids. (B) Near absence of tmem106bb mRNA in 3-dpf tmem106bb−/− KO embryos compared with wild-type tmem106bb+/+ embryos as revealed by RT-qPCR analysis (n = 3 samples/group with 20 embryos in each sample). (C) Comparison of morphology and length of 3-month adult tmem106bb−/− and compared with wild-type tmem106bb+/+ (n = 25–38 per group). **p < .01, ns, no significance; Data were shown as mean ± SD; Statistical analysis was performed using a Shapiro–Wilk test for samples with normal distribution and paired (B) or unpaired (C) Student's t tests with two tails.