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Fig. 5 Mn exposure increases slc39a11 expression in zebrafish, and slc39a11 Mut zebrafish have increased Mn content. (A to C) Summary of slc39a11 mRNA measured in untreated (Ctr) WT zebrafish embryos and embryos treated with either 100 μM MnCl2 or the chelator EDTA (A), 100 μM ZnSO4 (B), or 100 μM ferric citrate (C). (D) Summary of Mn, Zn, and Fe content measured in WT and slc39a11 Mut zebrafish embryos. (E to G) Summary of Mn (E), Zn (F), and Fe (G) content measured in male and female WT and Mut zebrafish adults. (H) Summary of sod2 mRNA measured in the muscle tissue of male and female WT and Mut zebrafish. (I) Fluorescence images of 4-HNE immunostaining (green) in muscle fibers of male and female WT and Mut zebrafish; the nuclei were counterstained with DAPI (blue). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.