|
Fig. 1 slc39a11 mutant zebrafish have an accelerated aging phenotype. (A) Summary of SLC39A11 mRNA measured in fibroblasts obtained from healthy controls and patients with Hutchinson–Gilford progeria syndrome (HGPS). (B) Alignment of the human, mouse, and zebrafish SLC39A11 proteins, with the 7 predicted transmembrane (TM) domains indicated; shown on the right are the homology scores obtained using ClustalW. (C) Strategy for generating slc39a11 mutant zebrafish. (D) Summary of slc39a11 mRNA measured in wild-type (WT) and slc39a11 mutant (Mut) zebrafish embryos. (E) Survival curves for male and female WT and Mut zebrafish (n ≥ 20 per group). (F) Representative images of adult (12-month-old) zebrafish. The red arrowheads indicate damaged fins. ***P < 0.001 and ****P < 0.0001.