Fig. 7 AE inhibits inflammatory cells recruitment in LPS-yolk microinjected zebrafish. (A) 3 dpf larvae (n = 15) were yolk-microinjected with PBS or LPS, and then treated with AE or DEX. The larvae were stained by H&E and taken pictures with a microscope, 20×. The black arrows represent inflammatory cells. (B) SB staining was performed at 9 hpi, 25×. (C) Statistical analysis of neutrophils in yolk sac of zebrafish larvae. (D) Whole-mount in situ hybridizations of mfap4 in zebrafish embryos at 9 hpi, 25×. (E) Statistical analysis of macrophages in yolk sac of zebrafish larvae. Data were represented as mean ± SD (n = 3), *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, no significance, compared with each? group. ##p < 0.01; ###p < 0.001; ####p < 0.0001; compared with Ctrl group.
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