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Fig. 6 The protective effect of AE on zebrafish larvae microinjected with LPS. (A) 3 dpf larvae (n = 20) were immersed with different concentrations of AE for 72 h. The survival rate of zebrafish larvae was observed under a microscope (B) Lateral view of 3 dpf zebrafish larvae treated with AE for 24 h. Scale bar, 500 ?m. (C) 3 dpf larvae (n = 15) were yolk-microinjected with PBS or LPS, and then treated with AE or DEX. Mortality was monitored until 72 hpi. (D) The heart-beating rate was counted at 9 hpi. (E) After 9 hpi the edema area of the yolk sac was tracked. Data were represented as mean ± SEM (n = 3), *p < 0.05; **p < 0.01; ***p < 0.001;****p < 0.0001; ns, no significance, compared with each?group. ##p < 0.01; ###p < 0.001; ####p < 0.0001; compared with Ctrl group.