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Fig. 6 Narrower notochords in stag2b mutants are rescued by additional stag1b mutation. (A,B) Examples of notochord width measurement using tbxta expression (magenta) and absence of sox2 expression (yellow) represented by tailbud images also shown in Fig. 5D,E. Scale bars: 20 μm. (C) Violin plots with overlaid box plots visualizing measurements of notochord width. The box plot limits indicate the interquartile range (IQR) between the first quartile (Q1) and the third quartile (Q3), the horizontal line inside the box represents the median (second quartile, Q2), and the whiskers extend to the smallest and largest values within 1.5× the IQR from Q1 and Q3, respectively, with any points outside this range considered outliers. The genotype and the number of embryos measured in each group are indicated on the x-axis. Significance was determined using an unpaired t-test: *P<0.05, ****P<0.0001. ns, not significant.