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Fig. 5 Local hepatocyte hyperplasia upon cryoinjury. (A) Schematic representation of the experiment workflow. (B-H) Liver sections from Tg(fabp10a:NLS-mKate) animals at the indicated stages, immunostained to detect proliferation (PCNA) and hepatocyte nuclei (mKate). Yellow arrowheads indicate proliferating hepatocytes. Cyan arrowheads indicate other cell types actively cycling. Dashed yellow line indicates the separation between healthy and injured liver parenchyma; IA, injured area; asterisks indicate spared hepatocytes surrounded by injured/necrotic tissue area. (I) Hepatocyte proliferation index in the border zone at the indicated stages (n=6, 5, 6, 5, 5, 4 and 5, left to right). Solid black line indicates the mean. P-values were calculated using one-way ANOVA followed by Tukey's multiple comparisons test. Scale bars: 25 µm.