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Fig. 4 Cryoinjury induces the local and transient infiltration of leukocytes. (A) Schematic representation of the experiment workflow. (B-H) Sections of livers from Tg(fabp10a:NLS-mKate) animals at the indicated stages, immunostained to detect hepatocyte nuclei (mKate) and leukocytes (Lcp1). Cyan arrowheads indicate low signal Lcp1+cells; dashed yellow line indicates the border zone; IA, injured area; yellow arrowheads indicate high signal Lcp1+ cells; asterisks indicate spared hepatocytes surrounded by injured/necrotic tissue area. (I) Quantification of the number of Lcp1+ cells in designated regions (n=6, 5, 6, 6, 5, 4 and 6, left to right). Solid black line indicates the mean. P-values wee calculated using one-way ANOVA followed by Tukey's multiple comparisons test. Scale bars: 25 µm.