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Fig. 2 V. cholerae T6SS-mediated antagonism towards E. coli induces host inflammation. (A) Fold change in the expression of cxcl8a, tnfa, il1b, mmp9 and cxcl18b in larvae infected with ~2000 CFU of WT or ΔT6SS V. cholerae relative to mock infected larvae at 6 hpi or 18 hpi. Data were pooled from three independent experiments, each with 12–15 larvae per time point. Significance was assessed using unpaired t-test on Log2 transformed values. (B) Fold change in the expression of cxcl8a, tnfa, il1b, mmp9 and cxcl18b in larvae coinfected with a 1:1 mixture (~1500 CFU each) of either E. coli and WT V. cholerae (Ec x Vc WT) or E. coli and ΔT6SS V. cholerae (Ec x Vc ΔT6) relative to mock infected larvae at 6 hpi or 18 hpi. Data were pooled from three independent experiments, each with 10–15 larvae per time point. Significance was assessed using unpaired t-test Log2 on transformed values. For all panels, bars indicate mean ± SEM. *p < 0.05, **p < 0.01, and ns (not significant). https://doi.org/10.1371/journal.ppat.1012384.g002