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Fig. 1 V. cholerae T6SS antagonism towards E. coli reduces host survival. (A) Illustration of zebrafish larvae indicating the site of bacterial injection (HBV) and downstream analysis workflow. (B) Enumeration of recovered V. cholerae (Vc) at 0, 6, or 24 hpi from larvae infected with ~2000 CFU of WT or ΔT6SS V. cholerae. Circles represent individual larvae. Data were pooled from 3 independent experiments, each with 3–4 larvae per time point. Significance was assessed using unpaired t-test on Log10 transformed values. (C) Survival curves of larvae infected with ~2000 CFU of WT or ΔT6SS V. cholerae. Data were pooled from three independent experiments, each with 12–26 larvae. Significance was assessed using log-rank Mantel-Cox test. (D) Enumeration of recovered E. coli or V. cholerae at 0, 6, or 24 hpi from zebrafish larvae coinfected with a 1:1 mixture (~1500 CFU each) of E. coli and WT V. cholerae (Ec x Vc WT) or E. coli and ΔT6SS V. cholerae (Ec x Vc ΔT6). Circles represent individual larvae. Data were pooled from three independent experiments, each with 3–5 larvae per time point. Significance was assessed using an unpaired t-test on Log10 transformed values. (E) Survival curves of larvae coinfected with a 1:1 mixture (~1500 CFU each) of either E. coli and WT V. cholerae (Ec x Vc WT) or E. coli and ΔT6SS V. cholerae (Ec x Vc ΔT6). Data were pooled from three independent experiments, each with 13–24 larvae. Significance was assessed by log-rank Mantel-Cox test. For all panels, bars indicate mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001, and ns (not significant). https://doi.org/10.1371/journal.ppat.1012384.g001