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Fig. 5 Overexpression of zebrafish aqp1a.1 rescues hyperglycaemia-caused narrowing of vascular diameter. A, Schematic representation of establishment of aqp1a.1-overexpressed transgenic line under Tg(fli1ep:EGFP-CAAX)ntu666 background. DNA construct and Tol2 transposase mRNA are co-injected at the 4-cell stage to establish a chimaera line, in which the mCherry-labelled Aqp1a.1 is mosaically expressed in the vascular system. B, Embryos are selected and immersed into high glucose solution from 2 to 3 dpf prior to imaging. C, Representative confocal images of ISVs in 3-dpf Tg(fli1ep:EGFP-CAAX::fli1ep:apq1.1-mCherry) double transgenic line (n = 40). D, Quantification of the lumen diameters in ISVs with or without Aqp1a.1 expression. Each data point represents an individual vessel diameter measurement of ISV, DA, and PCV. Five fishes are analysed for each group. Data are shown as mean ± s.e.m. A two-tailed, unpaired Student's t-test is applied. ****P < 0.0001. Scale bar, 100 μm.