Figure 2

Figure 2

Chemical screening using Tg(drl:hoxa9) identified compounds that restored myeloid differentiation. (A) A schematic representation of the chemical screening process. The effects of compounds on myeloid differentiation were examined by WISH of mpx, and Lef was used as a positive control. (B) Summary of primary and secondary screenings. (C) Tg(drl:hoxa9) embryos were treated with DMSO; Lef; or oxcarbazepine, khellin, ethacrynic acid, and alendronate and then assayed by WISH of mpx at 3 dpf. A green arrow indicates the staining signal. (D) Quantification of WISH results in (C). (E) Sibling and Tg(drl:hoxa9) embryos were treated with DMSO, Lef, oxcarbazepine, khellin, ethacrynic acid, and alendronate and stained for macrophages with NR at 5 dpf. (F) Quantification of results in (E). Results in (D,F) are represented as mean ± SEM, t-test; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p< 0.0001. NR, neutral red; Lef, leflunomide.