ZFIN Image: Wei et al., 2024, Figure 1

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Figure 1

Myeloid development is arrested in Tg(drl:hoxa9) zebrafish embryos. (A) Schematic representation of the drl:hoxa9-2A-GFP reporter gene and the DIC and fluorescent images of Tg(drl:hoxa9) embryos. GFP is expressed in the lateral plate mesoderm and derived hematopoietic tissues at different stages. (B) RT-qPCR analysis of hoxa9 expression in Tg(drl:hoxa9) embryos compared to sibling embryos. Results are represented as mean ± SEM, t-test; ** p < 0.01, **** p< 0.0001. (C,D) WISH of cmyb (myeloid progenitor cells), mpx (granulocytes), and mfap4 (macrophages) in Tg(drl:hoxa9) and sibling embryos at 3 dpf. Neutral red (NR) and Sudan black (SB) staining of macrophages and neutrophils in embryos at 5 dpf. A green arrow indicates the staining signal. drl, draculin; WISH, whole mount in situ hybridization; hpf, hours post-fertilization; dpf, days post-fertilization.

Acknowledgments

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