Figure 5

Figure 5

tg(olig2:EGFP) transgenic zebrafish reporter line injected with lrrk2 MO exhibited a reduction of Olig2 signal. (a) A pool of fifty embryos at 48 hpf injected with std or lrrk2 MO were subjected to immunoblotting using Lrrk2 and Gapdh antibodies. (b) Representative images of tg(olig2:EGFP) injected with std or lrrk2 MO were acquired by using an AXIOZOOM V16 ZEISS fluorescence microscope. (c) Quantification of fluorescence intensity of tg(olig2:EGFP) embryos at 48 hpf injected with std or lrrk2 MO calculated as the average of fluorescence intensity pixels ± SEM from three independent experiments (total embryos analyzed: std MO = 34 and lrrk2 MO = 31). Data were analyzed by using an unpaired t-test, **** p < 0.0001.