ZFIN Image: Fullone et al., 2024, Figure 5

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Figure 5

Analysis of ERK and STAT3 activation in CHO cells. (A,B) Analysis of ERK1/2 phosphorylation in CHO cells transfected with zPKR1 or hPKR1. Densitometric plots show pERK1/2 and ERK1/2 protein levels 10 min after treatment with zPK2 or hPK2 (100 nM). The bar graphs show the pERK1/2/ERK1/2 ratio and the percentage increase compared to unstimulated cells (CTRL). (C,D) Densitometric plots show phospho-STAT3 (pSTAT3) and STAT3 protein levels in CHO cells expressing zPKR1 or hPKR1 after 1 h of treatment with zPK2 or hPK2 (100 nM). Data are presented as the ratio of pSTAT3 to total STAT3 protein and plotted as a percentage increase compared to CTRL. The bars show the mean values ± SEM of the three experimental conditions. For statistical analysis, a one-way ANOVA followed by Tukey’s test for multiple comparisons was used. * p < 0.05, ** p < 0.01 versus CTRL.

Acknowledgments

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