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Fig. 3 Relative transcript levels of paraoxonase 1 (pon1) and peroxisome proliferator–activated receptor gamma (pparg) in 3.5 h postfertilization (hpf) to 5 days postfertilization unexposed zebrafish larvae over 11 time points and 1.5 hpf fertilized and unfertilized zebrafish embryos. The graphs for pon1 (A) and pparg (B) show log2 relative quantities of transcript levels normalized to reference gene transcript levels and expressed relative to the time point with the lowest transcript level. The red line depicts the locally estimated scatterplot smoothing (loess) fit, and the dashed blue line depicts the 95% confidence interval of the loess fit. Critical points indicate local minima or maxima in the best-fit curve. When the confidence intervals of the estimated best-fit function contain 0 at a specific age, that age is considered a critical point. See Vergauwen et al. (2018) for more details on the analysis. Fold changes of (C) pon1 (n = 8) and (D) pparg (n = 8) gene transcript levels normalized to reference gene transcript levels are shown as mean ± SD. *Statistically significant difference. Exposure to metabolism-disrupting chemicals affects the transcript levels of lipid metabolism genes. CI = confidence interval; dpf = days postfertilization.