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Fig. 5 Vipa mRNA and peptide are detected in zebrafish testis. In situ hybridization using vipa antisense riboprobe (A, B) and sense riboprobe (C) on sections from WT testis. Arrows point to cells where the positive signal was observed with the antisense riboprobe. Immunostaining of WT (D, E) and vipa−/− (F) testis with anti-Vipa precursor, and WT with preimmune serum (G). LC, Leydig cells; Ps, prespermatogonia; Spc I, primary spermatocytes; Spg, spermatogonia; Sptd, spermatids.
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