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Fig. 3 qPCR results. (A) Expression of the osteoblast-related markers osteocalcin (bglap) and collagen type I (col1a1a) is upregulated in LP animals compared to controls. Other osteoblast markers, i.e. osteonectin (sparc), osteopontin (spp1), and collagen type X (col10a1a), do not show differential expression between the three dietary groups. (B) Expression of the mineralization-related markers ectonucleotide pyrophosphatase phosphodiesterase 1 (enpp1), ectonucleoside triphosphate/diphospho-hydrolase 5 (entpd5), and tissue non-specific alkaline phosphatase (alpl) is significantly upregulated in LP zebrafish compared to controls. (C) Expression of the P-associated markers phosphatase orphan 1 (phospho1) and sclerostin (sost) is upregulated in LP zebrafish compared to controls. Phosphate regulating gene with homologies to endopeptidases on the X chromosome (phex) is not differentially expressed. In all panels, values are reported with Box and Whisker plots: the midline in each box is indicative of the median, whereas min and max values are shown with whiskers. Individual data points and P values are shown.