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Fig. 6 IER5L depletion elicits molecular alterations consistent with PP2A regulation. a Volcano plot representation of the phosphopeptides altered upon IER5L silencing by shScramble (shScr) or sh2 IER5L (shIER5L) transduction. The proteins inferred from the altered phosphopeptides that are reported targets of PP2A are highlighted. b Functional enrichment analysis of the proteins inferred from the differentially phosphorylated peptides upon IER5L silencing by Reactome. c Analysis of crystal violet staining after a 24-h treatment with 20 nM okadaic acid. The absorbance was normalized to Vehicle (Veh). A one-sample t-test was applied for statistical analysis (n = 4). d Analysis of the expression of the indicated genes by qRT-PCR upon a 24-h treatment with 20 nM okadaic acid. The levels are normalized to GAPDH and Vehicle (Veh). The dotted line represents the normalized value of the Veh data. A one-sample t-test was applied for statistical analysis (n = 3).