Fig. 6

Fig. 6

Cerebral (A, B) and ocular cerebral parasite load (C, D) of dams (A, C) and non-pregnant mice (B, D) infected with 100 oocysts of TgShSp1 strain and treated with BKI-1708 at 20 mg/kg/day for 5 days, or with corn oil as placebo (Positive control). Negative control was not infected and treated with corn oil. Eyes and brains were removed after euthanasia and T. gondii DNA was extracted and quantified by real-time PCR. Values are shown as box plots. Parasite burden between groups were compared using the Kruskal-Wallis test, followed by the Mann-Whitney-U test. Differences between BKI-1708-treated with 20 mg/kg and non-treated groups were statistically significant in non-pregnant mice and dams. (A) *P = 0.0328; **P = 0.0042, ***P = 0.0003; (B) *P = 0.0018; (C) **P = 0.0042; ***P = 0.0001; (D) **P = 0.005.